Anticorps Polyclonal de lapin anti-APOE

• Phare
• Validé par KD/KO

APOE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 18254-1-AP

Synonymes

Apo E, Apo-E, apoe4, apolipoprotein E, ApolipoproteinE

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Galerie de données de validation

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  WB analysis of HepG2 using 18254-1-AP

  WB result of APOE antibody (18254-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOE transfected HepG2 cells.

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  WB analysis using 18254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18254-1-AP (APOE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 18254-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18254-1-AP (APOE antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 18254-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18254-1-AP (APOE antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18254-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (18254-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HuH-7, plasma humain
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 8 publications below

Informations sur le produit

18254-1-AP cible APOE dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APOE Protéine recombinante Ag13070
• Nom complet: Apolipoprotein E
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34-38 kDa
• Numéro d’acquisition GenBank: BC003557
• Symbole du gène: APOE
• Identification du gène (NCBI): 348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight. APOE can be detected 43kDa and 69kDa dimers (PMID: 9831633).

Publications

Species	Application	Title
human	IHC	Br J Cancer; A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.; Authors - Xiaohui Liu
human	IHC	Transl Oncol; Multi-omics analysis-based macrophage differentiation-associated papillary thyroid cancer patient classifier; Authors - Hanlin Sun
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Inducible global knockout of surfeit locus protein 4 in adult mice results in hypolipidemia, intestinal lipid accumulation, liver injury, and increased mortality; Authors - Wei Chen
human	WB	J Virol; SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway.; Authors - Da-Yuan Chen
human	WB	bioRxiv; SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.; Authors - Da-Yuan Chen
human	IF	Stem Cell Res Ther; Single-cell sequencing of facial adipose tissue unveils FKBP5 as a therapeutic target for facial infiltrating lipomatosis; Authors - Hongrui Chen

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