Anticorps Polyclonal de lapin anti-APOH

APOH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 11892-1-AP

Synonymes

Apolipoprotein H, B2G1, ApolipoproteinH, Apo-H, Apo H

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Galerie de données de validation

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  WB analysis using 11892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11892-1-AP (Apolipoprotein H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11892-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11892-1-AP (Apolipoprotein H antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 11892-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11892-1-AP (Apolipoprotein H antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11892-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11892-1-AP (Apolipoprotein H antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11892-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein H antibody (11892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu testiculaire de souris
• Résultats positifs en IHC: human normal colon, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11892-1-AP cible APOH dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APOH Protéine recombinante Ag2500
• Nom complet: apolipoprotein H (beta-2-glycoprotein I)
• Masse moléculaire calculée: 345 aa, 38 kDa
• Poids moléculaire observé: 43-55 kDa
• Numéro d’acquisition GenBank: BC026283
• Symbole du gène: APOH
• Identification du gène (NCBI): 350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins. ApoH is a 54 kDa plasma glycoprotein.

Publications

Species	Application	Title
human	IHC	Nat Commun; ATRX, DAXX or MEN1 mutant pancreatic neuroendocrine tumors are a distinct alpha-cell signature subgroup.; Authors - Chang S Chan
human	IP	Neuropharmacology; Anti-β2GPI/β2GPI induces neutrophil extracellular traps formation to promote thrombogenesis via the TLR4/MyD88/MAPKs axis activation.; Authors - Caijun Zha
human,mouse	WB	J Biol Chem; Development of betabodies: the next generation of phosphatidylserine targeting agents; Authors - Natalie Z Phinney
human	WB	Cancer Med; Identification of novel pQTL-SNPs associated with lung adenocarcinoma risk: A multi-stage study; Authors - Yutong Wu

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