Anticorps Polyclonal de lapin anti-APOL1

• Phare
• Validé par KD/KO

APOL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 11486-2-AP

Synonymes

APO L, APOL, APOL I, APOL1, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

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Galerie de données de validation

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  WB analysis of HepG2 using 11486-2-AP

  WB result of APOL1 antibody (11486-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOL1 transfected HepG2 cells.

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  WB analysis using 11486-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 11486-2-AP

  human blood were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 11486-2-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11486-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 11486-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11486-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11486-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11486-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 11486-2-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11486-2-AP

  IP Result of anti-APOL1 (IP:11486-2-AP, 3ug; Detection:11486-2-AP 1:500) with HepG2 cells lysate 6000ug.

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  IP experiment of human plasma using 11486-2-AP

  IP Result of anti-APOL1 (IP:11486-2-AP, 4ug; Detection:11486-2-AP 1:500) with human plasma lysate 4000ug.

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  IHC staining of human kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11486-2-AP (APOL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11486-2-AP (APOL1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11486-2-AP (APOL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11486-2-AP

  Immunofluorescent analysis of () fixed HepG2 cells using 11486-2-AP (APOL1 antibody) at dilution of 1:25.

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  IF Staining of HEK-293 using 11486-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using APOL1 antibody (11486-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 11486-2-AP

  1X10^6 HepG2 cells were stained with 0.2ug APOL1 antibody (11486-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A2780, cellules A549, cellules HeLa, cellules HepG2, cellules MCF-7, cellules SKOV-3, cellules SW-1990, plasma humain
• Résultats positifs en IP: cellules HepG2, tissu plasmatique humain
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain, tissu pancréatique humain, tissu rénal de souris, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

11486-2-AP cible APOL1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APOL1 Protéine recombinante Ag2016
• Nom complet: apolipoprotein L, 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC017331
• Symbole du gène: APOL1
• Identification du gène (NCBI): 8542
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous APOL1 of 39-45 kDa in blood lysate.

Publications

Species	Application	Title
human	WB	J Exp Med; Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model.; Authors - Molina-Portela Maria Pilar MP
human	WB	Proc Natl Acad Sci U S A; Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.; Authors - Thomson Russell R
	WB	Proc Natl Acad Sci U S A; Evolution of the primate trypanolytic factor APOL1.; Authors - Russell Thomson
human	WB	J Am Soc Nephrol; Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes.; Authors - Nidhi Gupta ; KD Validated
human	WB,IF	J Am Soc Nephrol; Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.; Authors - Suzie J Scales ; KO Validated
human	IHC	Br J Cancer; A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.; Authors - Xiaohui Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (02-04-2023)

Good antibody for detect apol1 protein

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: 293T

APOL1 Antibody Immunofluorescence validation (1 to 200 dilution) in 293T (Cat no:11486-2-AP)

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