Anticorps Recombinant de lapin anti-APOM

APOM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

3O19

N° de cat : 82871-1-RR

Synonymes

HSPC336, G3A, Apo-M, Apolipoprotein M, Apo M

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Galerie de données de validation

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  WB analysis using 82871-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82871-1-RR (APOM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 82871-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (82871-1-RR, Clone: 3O19 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HepG2 using 82871-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug APOM Recombinant antibody (82871-1-RR, Clone:3O19) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82871-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82871-1-RR against Human APOM were performed. The affinity constant is 0.694 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, plasma humain
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82871-1-RR cible APOM dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: APOM Protéine recombinante Ag3048
• Nom complet: apolipoprotein M
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: BC020683
• Symbole du gène: APOM
• Identification du gène (NCBI): 55937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).

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