Anticorps Monoclonal anti-APP

• Phare
• Validé par KD/KO

APP Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(26)

CloneNo.

5C2A1

Avis (1)

N° de cat : 60342-1-Ig

Synonymes

amyloid precursor protein, Alzheimer disease amyloid A4 protein homolog, AD1, AB42, AB40

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Galerie de données de validation

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  WB analysis using 60342-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 60342-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60342-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 60342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 60342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 60342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (Beta Amyloid antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 60342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (Beta Amyloid antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules HEK-293, cellules Y79, tissu cérébral de lapin, tissu cérébral de rat
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Informations sur le produit

60342-1-Ig cible APP dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat

• Réactivité: Humain, Lapin, porc, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APP Protéine recombinante Ag0769
• Nom complet: amyloid beta (A4) precursor protein
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC004369
• Symbole du gène: APP
• Identification du gène (NCBI): 351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1).

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression; Authors - Rui Zhou
mouse	WB,IHC,IP	Aging Cell; Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model.; Authors - Qi Shen
human	WB	Protein Cell; Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model.; Authors - Xiaoyan Xu
human	WB	Cell Rep; CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.; Authors - Yang-Ping Shentu
mouse	WB,IF	Aging (Albany NY); Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease.; Authors - Chao Zhou
mouse	IF	J Ethnopharmacol; Lancao decoction alleviates Alzheimer's disease: depending on activating CaMKII to protect neuronal refunction by reducing β-amyloid in the hippocampus; Authors - Lei Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jinwei (Verified Customer) (10-01-2021)

The Beta Amyloid Monoclonal antibody works fine.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293

APP Antibody Western Blot validation (1:5000 dilution) in HEK293 (Cat no:60342-1-Ig)

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