Anticorps Polyclonal de lapin anti-APPBP2

APPBP2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12409-1-AP

Synonymes

APP BP2, APPBP2, HS.84084, KIAA0228, PAT1

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Galerie de données de validation

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  WB analysis using 12409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12409-1-AP (APPBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12409-1-AP (APPBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12409-1-AP cible APPBP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APPBP2 Protéine recombinante Ag3092
• Nom complet: amyloid beta precursor protein (cytoplasmic tail) binding protein 2
• Masse moléculaire calculée: 585 aa, 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC018121
• Symbole du gène: APPBP2
• Identification du gène (NCBI): 10513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Amyloid proteinbinding protein 2 (APPBP2) is a hydrophilic tubulin that interacts with microtubules and is functionally involved in beta-amyloid precursor protein (APP) transport and/or processing. Based on the regulation of microtubules and APP, APPBP2 is found to be involved in the oncogenesis of various types of cancers, such as breast cancer, ovarian clear cell adenocarcinomas, desmoplastic medulloblastomas and neuroblastomas. Recently, Studies have demonstrated that APPBP2 is a potential target for diagnosis and therapeutic intervention in non-small cell lung cancer (NSCLC) (PMID: 31105033).

Publications

Species	Application	Title
mouse	WB	Diabetes; PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ; Authors - Yi Zhong