Anticorps Monoclonal anti-AQP1

AQP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

2E5D6

N° de cat : 66805-1-Ig

Synonymes

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

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Galerie de données de validation

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  WB analysis of human heart using 66805-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 66805-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 66805-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66805-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66805-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human kidney using 66805-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AQP1 antibody (66805-1-Ig, Clone: 2E5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu de muscle squelettique humain, tissu rénal de porc
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 5 publications below

Informations sur le produit

66805-1-Ig cible AQP1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AQP1 Protéine recombinante Ag14093
• Nom complet: aquaporin 1 (Colton blood group)
• Masse moléculaire calculée: 269 aa, 29 kDa
• Poids moléculaire observé: 28 kDa, 38-40 kDa
• Numéro d’acquisition GenBank: BC022486
• Symbole du gène: AQP1
• Identification du gène (NCBI): 358
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)

Publications

Species	Application	Title
human	IF	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	IF	Adv Sci (Weinh); Behind the Indolent Facade: Uncovering the Molecular Features and Malignancy Potential in Lung Minimally Invasive Adenocarcinoma by Single-Cell Transcriptomics; Authors - Xin Zhang
human	IF	Clin Transl Med; Single-cell RNA sequencing of pediatric renal tissues revealed the potential relationship between immunoglobulin A nephropathy and immunoglobulin A vasculitis with nephritis; Authors - Qing Ye
mouse	IF	J Cell Mol Med; HBSP inhibits tubular cell pyroptosis and apoptosis, promotes macrophage M2 polarization, and protects LPS-induced acute kidney injury; Authors - Lili Huang
human	IF	STAR Protoc; Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature; Authors - Léa Pechtimaldjian

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