Anticorps Polyclonal de lapin anti-AQP2

AQP2 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 29386-1-AP

Synonymes

Aquaporin 2, AQP-2, Aquaporin2

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Galerie de données de validation

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  WB analysis of mouse kidney using 29386-1-AP

  37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29386-1-AP (AQP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 29386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29386-1-AP (AQP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 29386-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29386-1-AP (AQP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 29386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP2 antibody (29386-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), AQP1 antibody (66805-1-Ig, Clone: 2E5D6, green).

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  IF Staining of mouse kidney using 29386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using AQP2 antibody (29386-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris incubé à 37 °C,
• Résultats positifs en IHC: tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,
• Résultats positifs en IF-Fro: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

29386-1-AP cible AQP2 dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AQP2 Protéine recombinante Ag29931
• Nom complet: aquaporin 2 (collecting duct)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 28-29, 35-38 kDa
• Numéro d’acquisition GenBank: NM_000486
• Symbole du gène: AQP2
• Identification du gène (NCBI): 359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.

Publications

Species	Application	Title
mouse	WB,IF	FASEB J; Circadian rhythm disruptions exacerbate inner ear damage in a murine endolymphatic hydrops model; Authors - Bo Pang
mouse	IF	Environ Pollut; Ozone exposure induced kidney damage in diabetic mice: the key role of lipid metabolism and water-electrolyte homeostasis; Authors - Yaru Zhang
mouse	IF	Diabetes Metab J; Kidney Gastrin/CCKBR Attenuates Type 2 Diabetes Mellitus by Inhibiting SGLT2-Mediated Glucose Reabsorption through Erk/NF-κB Signaling Pathway; Authors - Xue Zhang
mouse	IF	Research (Wash D C); Single-Cell RNA Sequencing Analysis Reveals the Role of Macrophage-Mediated CD44-AKT-CCL2 Pathways in Renal Tubule Injury during Calcium Oxalate Crystal Formation; Authors - Xi Jin
mouse	IHC	EMBO Mol Med; Melanin-like nanoparticles slow cyst growth in ADPKD by dual inhibition of oxidative stress and CREB; Authors - Yongzhan Sun

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