Anticorps Polyclonal de lapin anti-ARF1

• Phare
• Validé par KD/KO

ARF1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (2)

N° de cat : 10790-1-AP

Synonymes

ADP ribosylation factor 1, ARF1

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Galerie de données de validation

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  WB analysis using 10790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10790-1-AP

  WB result of ARF1 antibody (10790-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HEK-293 cells.

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  WB analysis of rat brain using 10790-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10790-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10790-1-AP

  WB result of ARF1 antibody (10790-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HeLa cells.

• 

  WB analysis of mouse brain using 10790-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 10790-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10790-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10790-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10790-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10790-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10790-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10790-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10790-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10790-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (10790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10790-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COS-7, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10790-1-AP cible ARF1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARF1 Protéine recombinante Ag1244
• Nom complet: ADP-ribosylation factor 1
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 18-21 kDa
• Numéro d’acquisition GenBank: BC009247
• Symbole du gène: ARF1
• Identification du gène (NCBI): 375
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment. (PMID: 7759471, PMID: 16042562).This antibody can bind ARFs for the close sequences.

Publications

Species	Application	Title
human	WB	Cell Host Microbe; A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.; Authors - Cheryl N Miller ; KD Validated
human	IF	EMBO J; Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase.; Authors - Shoken Lee
human	WB	Theranostics; Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.; Authors - Hui-Fang Hu
human	WB	BMC Biol; ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.; Authors - Xihua Yue
human	WB	Fertil Steril; Pathogenic variants in TSC2 might cause premature ovarian insufficiency through activated mTOR induced hyperactivation of primordial follicles; Authors - Bingying Xu
human	WB	Sci Rep; The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.; Authors - Xiaobo Lei ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Cristian (Verified Customer) (04-26-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

ARF1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:10790-1-AP)

FH

Boyan (Verified Customer) (01-25-2019)

By IF, PFA fixation, this antibody gave a Actin-like staining, but in literature and also its data sheet, it should be localised to the Golgi.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: MEF