Anticorps Polyclonal de lapin anti-ARF5

ARF5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 15281-1-AP

Synonymes

ADP ribosylation factor 5, ARF5

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Galerie de données de validation

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  WB analysis of MCF-7 using 15281-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15281-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15281-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 15281-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15281-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 15281-1-AP

  IP result of anti-ARF5 (IP:15281-1-AP, 4ug; Detection:15281-1-AP 1:500) with MCF-7 cells lysate 2840 ug.

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  IHC staining of human breast cancer using 15281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15281-1-AP (ARF5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15281-1-AP (ARF5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15281-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15281-1-AP (ARF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules PC-12, cellules Raji, tissu cérébral de souris, tissu hépatique humain
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

15281-1-AP cible ARF5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARF5 Protéine recombinante Ag7400
• Nom complet: ADP-ribosylation factor 5
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC003043
• Symbole du gène: ARF5
• Identification du gène (NCBI): 381
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Publications

Species	Application	Title
human	WB	J Physiol Biochem; Rab11-FIP4 interacts with ARF5 to promote cancer stemness in hepatocellular carcinoma; Authors - Feifeng Song ; KD Validated