Anticorps Monoclonal anti-Arfaptin-1

Arfaptin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2A10B3

N° de cat : 67461-1-Ig

Synonymes

ARFIP1, Arfaptin 1, ADP-ribosylation factor-interacting protein 1, 2A10B3

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Galerie de données de validation

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  WB analysis using 67461-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67461-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67461-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67461-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 67461-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 67461-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 67461-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse kidney using 67461-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67461-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A549 using 67461-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67461-1-Ig cible Arfaptin-1 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Arfaptin-1 Protéine recombinante Ag12097
• Nom complet: ADP-ribosylation factor interacting protein 1
• Masse moléculaire calculée: 373 aa, 42 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC103759
• Symbole du gène: Arfaptin-1
• Identification du gène (NCBI): 27236
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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