Anticorps Polyclonal de lapin anti-Arginase-2

• Phare
• Validé par KD/KO

Arginase-2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 14825-1-AP

Synonymes

ARG2, ARG 2, EC:3.5.3.1, Arginase-2, mitochondrial, arginase, type II

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Galerie de données de validation

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  WB analysis of Caco-2 using 14825-1-AP

  WB result of ARG2 antibody (14825-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arginase-2 transfected Caco-2 cells.

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  WB analysis using 14825-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 14825-1-AP (Arginase-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14825-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14825-1-AP (Arginase-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14825-1-AP (ARG2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

14825-1-AP cible Arginase-2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Arginase-2 Protéine recombinante Ag6609
• Nom complet: arginase, type II
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39-42 kDa
• Numéro d’acquisition GenBank: BC001350
• Symbole du gène: ARG2
• Identification du gène (NCBI): 384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)

Publications

Species	Application	Title
mouse	IF	Circulation; Deletion of Macrophage Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Accelerates Atherosclerosis Regression and Increases C-C Chemokine Receptor Type 7 (CCR7) Expression in Plaque Macrophages.; Authors - Paul A Mueller
mouse	WB	J Lipid Res; Macrophage-derived apoESendai suppresses atherosclerosis while causing lipoprotein glomerulopathy in hyperlipidemic mice.; Authors - Hagai Tavori
mouse	WB	Biomed Pharmacother; PMID: 30889486; Authors - Lu Gao
mouse	WB	Biochim Biophys Acta; Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model.; Authors - Terry Jo V Bichell
	IHC	Biol Reprod; Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.; Authors - Katherine M Halloran
human	WB	Life Sci; Ornithine is a key mediator in hyperphosphatemia-mediated human umbilical vein endothelial cell apoptosis: Insights gained from metabolomics.; Authors - Rong Zhou

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