Anticorps Polyclonal de lapin anti-ARHGEF1

ARHGEF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (3)

N° de cat : 11363-1-AP

Synonymes

ARHGEF1, GEF1, LBCL2, LSC, P115 RHOGEF, p115RhoGEF, SUB1.5

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Galerie de données de validation

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  WB analysis of HEK-293 using 11363-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 11363-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11363-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11363-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11363-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11363-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11363-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11363-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11363-1-AP

  IP Result of anti-ARHGEF1 (IP:11363-1-AP, 4ug; Detection:11363-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon using 11363-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 11363-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11363-1-AP

  Immunofluorescent analysis of HeLa cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 11363-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, tissu cérébral humain, tissu splénique de souris, tissu splénique humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

11363-1-AP cible ARHGEF1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARHGEF1 Protéine recombinante Ag1905
• Nom complet: Rho guanine nucleotide exchange factor (GEF) 1
• Masse moléculaire calculée: 912 aa, 102 kDa
• Poids moléculaire observé: 102-110 kDa
• Numéro d’acquisition GenBank: BC011726
• Symbole du gène: ARHGEF1
• Identification du gène (NCBI): 9138
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nature; Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.; Authors - Silvana Konermann
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian
human	WB	Mol Biol Cell; The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.; Authors - P V Ryder
mouse	CoIP,WB	Nat Commun; Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis; Authors - Yaping Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (12-29-2023)

Worked well for IP using 6ug Ab and WB 1:1500

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioma

FH

can (Verified Customer) (12-17-2019)

Very good antibody!

• Applications: Immunoprecipitation,
• Cell Tissue Type: blood vascular cell

FH

Ni (Verified Customer) (09-26-2019)

Very successful in immunoprecipitating p115RhoGEF from transfected cells.

• Applications: Immunoprecipitation,
• Primary Antibody Dilution: 2 ug /reaction