Anticorps Polyclonal de lapin anti-ARHGEF5

ARHGEF5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11379-1-AP

Synonymes

ARHGEF5, GEF5, Oncogene TIM, P60, p60 TIM, TIM, TIM1

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Galerie de données de validation

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  WB analysis of MCF-7 using 11379-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11379-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11379-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 11379-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11379-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11379-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11379-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11379-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 11379-1-AP

  IP Result of anti-ARHGEF5 (IP:11379-1-AP, 4ug; Detection:11379-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human lung cancer using 11379-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11379-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11379-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11379-1-AP (ARHGEF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HEK-293, cellules HeLa, tissu hépatique de souris, tissu hépatique humain, tissu rénal de rat, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11379-1-AP cible ARHGEF5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARHGEF5 Protéine recombinante Ag1892
• Nom complet: Rho guanine nucleotide exchange factor (GEF) 5
• Masse moléculaire calculée: 177 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC010046
• Symbole du gène: ARHGEF5
• Identification du gène (NCBI): 7984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB, IF	J Biol Chem; Odontogenic ameloblast-associated protein (ODAM) Mediates Junctional Epithelium Attachment to Tooth via Integrin-ODAM-Rho guanine nucleotide exchange factor 5 (ARHGEF5)-Ras homolog gene family member A (RhoA) Signaling.; Authors - Hye-Kyung Lee
human	IHC	Oncol Rep; Co-expression of Rho guanine nucleotide exchange factor 5 and Src associates with poor prognosis of patients with resected non-small cell lung cancer.; Authors - He Ping P
human	WB	Cell Biochem Funct; ODAM inhibits RhoA-dependent invasion in breast cancer.; Authors - Hye-Kyung Lee