Anticorps Polyclonal de lapin anti-ARL1

• Phare
• Validé par KD/KO

ARL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 16012-1-AP

Synonymes

ADP ribosylation factor like 1, ADP-ribosylation factor-like protein 1

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Galerie de données de validation

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  WB analysis using 16012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16012-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16012-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

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  IP experiment of Neuro-2a using 16012-1-AP

  IP result of anti-ARL1 (IP:16012-1-AP, 4ug; Detection:16012-1-AP 1:4000) with Neuro-2a cells lysate 2400 ug.

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  IHC staining of mouse brain using 16012-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16012-1-AP (ARL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 16012-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16012-1-AP (ARL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16012-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARL1 antibody (16012-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules C6, cellules HeLa, cellules HepG2, cellules K-562, cellules Neuro-2a, tissu hépatique de souris
• Résultats positifs en IP: cellules Neuro-2a,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

16012-1-AP cible ARL1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARL1 Protéine recombinante Ag8770
• Nom complet: ADP-ribosylation factor-like 1
• Masse moléculaire calculée: 181 aa, 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC007000
• Symbole du gène: ARL1
• Identification du gène (NCBI): 400
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
human	WB	Nature; FSP1 is a glutathione-independent ferroptosis suppressor.; Authors - Sebastian Doll
human	WB,IP	Nat Protoc; Proteome-wide analysis of protein lipidation using chemical probes: in-gel fluorescence visualization, identification and quantification of N-myristoylation, N- and S-acylation, O-cholesterylation, S-farnesylation and S-geranylgeranylation.; Authors - Wouter W Kallemeijn
human	WB	Nat Commun; Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins.; Authors - Noura Alzahofi
human	WB	J Cell Biol; ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.; Authors - Morié Ishida ; KO Validated
human	IF	Cell Rep; Role for the IFT-A Complex in Selective Transport to the Primary Cilium.; Authors - Wenxiang Fu

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