Anticorps Polyclonal de lapin anti-ARL2

• Phare
• Validé par KD/KO

ARL2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10232-1-AP

Synonymes

ADP ribosylation factor like 2, ARFL2, ARL2

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Galerie de données de validation

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  WB analysis of mouse liver using 10232-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10232-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10232-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10232-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 10232-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10232-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10232-1-AP (ARL2 antibody) at dilution of 1:100 (under 25x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules Y79, tissu pulmonaire de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

10232-1-AP cible ARL2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARL2 Protéine recombinante Ag0334
• Nom complet: ADP-ribosylation factor-like 2
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: BC002530
• Symbole du gène: ARL2
• Identification du gène (NCBI): 402
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ADP-ribosylation factor (ARF) genes are small GTP-binding proteins of the RAS superfamily. ADP-ribosylation factor-like 2 (ARL2) is a member of a functionally distinct group of ARF-like genes. ARL2 interacts with the tubulin-specific chaperone protein known as cofactor D. ARL2 downregulates the tubulin GAP activity of cofactor C, D, and E, and inhibits the binding of D to native tubulin.

Publications

Species	Application	Title
rat	WB	J Biol Chem; MicroRNA-15b modulates cellular ATP levels and degenerates mitochondria via Arl2 in neonatal rat cardiac myocytes.; Authors - Nishi Hitoo H ; KD Validated
mouse	WB	J Biol Chem; Selective Impairment of a Subset of Ran-GTP-binding Domains of Ran-binding protein 2 (Ranbp2) Suffices to Recapitulate the Degeneration of the Retinal Pigment Epithelium (RPE) Triggered by Ranbp2 Ablation.; Authors - Hemangi Patil
mouse	WB,IF	J Cell Biochem; GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis.; Authors - Chun-Xiang Zhou
mouse	WB	Cell Cycle; GTPase-activating protein Elmod2 is essential for meiotic progression in mouse oocytes.; Authors - Chun-Xiang Zhou
human	WB,IF	Invest Ophthalmol Vis Sci; Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis.; Authors - Mori Naoki N
mouse	IF	PeerJ; Effective protein inhibition in intact mouse oocytes through peptide nanoparticle-mediated antibody transfection.; Authors - Ruichao Li