Anticorps Polyclonal de lapin anti-ARL2BP

• Phare
• Validé par KD/KO

ARL2BP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 10090-2-AP

Synonymes

RP66, BART1, BART, ARF-like 2-binding protein, ADP-ribosylation factor-like protein 2-binding protein

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Galerie de données de validation

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  WB analysis of A549 using 10090-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10090-2-AP (ARL2BP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10090-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10090-2-AP (ARL2BP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10090-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10090-2-AP (ARL2BP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

10090-2-AP cible ARL2BP dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARL2BP Protéine recombinante Ag0138
• Nom complet: ADP-ribosylation factor-like 2 binding protein
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC003087
• Symbole du gène: ARL2BP
• Identification du gène (NCBI): 23568
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARF-like proteins (ARLs) belong to a group of incompletely characterized members in the ARF family of RAS-related GTPases. A 19-kDa protein (BART, Binder of Arl Two) was identified to bind specifically to ARL2.GDP with high affinity but not with ARL2.GDP. In human, the 489-base pair BART open reading frame encodes a novel 163-amino acid protein with a predicted molecular mass of 18,822 Da. Data from Northern and Western analyses indicated BART is expressed in many tissues.

Publications

Species	Application	Title
mouse	WB,IF	Am J Hum Genet; Mutations in ARL2BP, Encoding ADP-Ribosylation-Factor-Like 2 Binding Protein, Cause Autosomal-Recessive Retinitis Pigmentosa.; Authors - Davidson Alice E AE ; KD Validated
human	WB	Cancer Res; Intracellular CD24 inhibits cell invasion by posttranscriptional regulation of BART through interaction with G3BP.; Authors - Keisuke Taniuchi
human	WB,IF	Neoplasia; BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1.; Authors - Taniuchi Keisuke K ; KD Validated
human	WB,IF	PLoS One; BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.; Authors - Taniuchi Keisuke K ; KD Validated
human	WB,IF	Invest Ophthalmol Vis Sci; Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis.; Authors - Mori Naoki N
human	WB,IF,IP	Int J Oncol; BART inhibits pancreatic cancer cell invasion by inhibiting ARL2-mediated RhoA inactivation.; Authors - Taniuchi Keisuke K

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