Anticorps Polyclonal de lapin anti-ARL4C

• Phare
• Validé par KD/KO

ARL4C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 10202-1-AP

Synonymes

ADP-ribosylation factor-like protein 4C, ADP-ribosylation factor-like protein 7, ADP-ribosylation factor-like protein LAK, ARL7

---

Galerie de données de validation

• 

  WB analysis of HUVEC using 10202-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 10202-1-AP (ARL4C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10202-1-AP

  WB result of ARL4C antibody (10202-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARL4C transfected SKOV-3 cells.

• 

  WB analysis of human brain using 10202-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10202-1-AP (ARL4C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HUVEC using 10202-1-AP

  IP result of anti-ARL4C (IP:10202-1-AP, 4ug; Detection:10202-1-AP 1:500) with HUVEC cells lysate 3600 ug.

• 

  IHC staining of human colon cancer using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HUVEC using 10202-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using ARL4C antibody (10202-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of A549 using 10202-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ARL4C antibody (10202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules HUVEC, tissu cérébral humain
• Résultats positifs en IP: cellules HUVEC,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HUVEC, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Informations sur le produit

10202-1-AP cible ARL4C dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARL4C Protéine recombinante Ag0246
• Nom complet: ADP-ribosylation factor-like 4C
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: BC001051
• Symbole du gène: ARL4C
• Identification du gène (NCBI): 10123
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Cell; A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.; Authors - Banaszynski Laura A LA
human	WB	Genome Med; Integrative epigenomic and high-throughput functional enhancer profiling reveals determinants of enhancer heterogeneity in gastric cancer.; Authors - Taotao Sheng ; KD Validated
mouse	WB,IF	Proc Natl Acad Sci U S A; CRL5-dependent regulation of the small GTPases ARL4C and ARF6 controls hippocampal morphogenesis.; Authors - Jisoo S Han ; KD Validated
human	WB,IHC	Front Pharmacol; Identification of biomarkers and potential drug targets in osteoarthritis based on bioinformatics analysis and mendelian randomization; Authors - Feng Cheng
human	WB,IHC	Cancer Sci; ARL4C is associated with initiation and progression of lung adenocarcinoma and represents a therapeutic target.; Authors - Kenji Kimura
human	WB,IF	iScience; Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.; Authors - Xin Chen ; KD Validated

{{ptg:RelatedPrimaryAntibodies}}