Anticorps Monoclonal anti-ARL8A/ARL8B

ARL8A/ARL8B Monoclonal Antibody for IF/ICC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, rat, souris

Applications

IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F4C3

N° de cat : CL488-68186

Synonymes

ARL8A, GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B

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Galerie de données de validation

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  IF Staining of HEK-293 using CL488-68186

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ARL8A/ARL8B antibody (CL488-68186, Clone: 2F4C3 ) at dilution of 1:200.

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  IF Staining of A549 using CL488-68186

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 ARL8A/ARL8B antibody (CL488-68186, Clone: 2F4C3 ) at dilution of 1:200.

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  IF Staining of Neuro-2a using CL488-68186

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CoraLite® Plus 488 ARL8A/ARL8B antibody (CL488-68186, Clone: 2F4C3 ) at dilution of 1:200.

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  FC experiment of C6 using CL488-68186

  1x10^6 C6 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Arl8a/Arl8b Monoclonal Antibody (CL488-68186, Clone:2F4C3)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HEK-293, cellules A549, cellules Neuro-2a
• Résultats positifs en FC (Intra): cellules C6,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-68186 cible ARL8A/ARL8B dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARL8A/ARL8B Protéine recombinante Ag10758
• Nom complet: ADP-ribosylation factor-like 8A
• Masse moléculaire calculée: 186 aa, 21 kDa
• Poids moléculaire observé: 18 kDa, 21 kDa
• Numéro d’acquisition GenBank: BC015408
• Symbole du gène: ARL8A
• Identification du gène (NCBI): 127829
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A/ARL8B can be recognized by this antibody.

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