Anticorps Polyclonal de lapin anti-ARPC1B

• Phare
• Validé par KD/KO

ARPC1B Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 28368-1-AP

Synonymes

Actin-related protein 2/3 complex subunit 1B, ARC41, p41 ARC, p41-ARC

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Galerie de données de validation

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  WB analysis of HeLa using 28368-1-AP

  WB result of ARPC1B antibody (28368-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARPC1B transfected HeLa cells.

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  WB analysis using 28368-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28368-1-AP (ARPC1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28368-1-AP

  IP result of anti-ARPC1B (IP:28368-1-AP, 4ug; Detection:28368-1-AP 1:1000) with HeLa cells lysate 1600 ug.

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  IHC staining of human colon cancer using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral de souris, tissu de cancer du poumon humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

28368-1-AP cible ARPC1B dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARPC1B Protéine recombinante Ag29001
• Nom complet: actin related protein 2/3 complex, subunit 1B, 41kDa
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC007555
• Symbole du gène: ARPC1B
• Identification du gène (NCBI): 10095
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARPC1B also known as ARC41, p41-ARC, or p40-ARC, is one of seven subunits of the human Arp2/3 protein complex, that belongs to the SOP2 family. ARPC1B localizes on centrosomes and has a distinct role in centrosomal homeostasis. ARPC1B is both a physiological activator and substrate of Aurora A kinase, and these interactions help to maintain mitotic integrity in mammalian cells. In recent resaeach, ARPC1B is selected as a candidate prediction marker for sensitivity of Choroidal malignant melanomas to radiotherapy.

Publications

Species	Application	Title
mouse,human	WB	Front Immunol; The overexpression of actin related protein 2/3 complex subunit 1B(ARPC1B) promotes the ovarian cancer progression via activation of the Wnt/β-catenin signaling pathway; Authors - Junning Huang
human	IHC	J Transl Med; A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer; Authors - Huixia Huang
human	WB,IHC	Mol Cell Probes; High expression of ARPC1B promotes the proliferation and Apoptosis of clear cell renal cell carcinoma cells, leading to a poor prognosis; Authors - Hongbo Wang ; KD Validated
mouse	WB	CNS Neurosci Ther; Integrated Mendelian Randomization and Single-Cell Transcriptomics Analysis Identifies Critical Blood Biomarkers and Potential Mechanisms in Epilepsy; Authors - Jianwei Shi

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