Anticorps Polyclonal de lapin anti-ARPC5L

ARPC5L Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 22025-1-AP

Synonymes

ARC16 2, ARPC5L

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Galerie de données de validation

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  WB analysis using 22025-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22025-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22025-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 22025-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22025-1-AP

  IP Result of anti-ARPC5L (IP:22025-1-AP, 4ug; Detection:22025-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 22025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 22025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cerebellum using 22025-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cerebellum using 22025-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 22025-1-AP

  Immunofluorescent analysis of MCF-7 cells using 22025-1-AP (ARPC5L antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of MCF-7 using 22025-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug ARPC5L antibody (22025-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral humain, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu de cervelet humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

22025-1-AP cible ARPC5L dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARPC5L Protéine recombinante Ag16881
• Nom complet: actin related protein 2/3 complex, subunit 5-like
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17-20 kDa
• Numéro d’acquisition GenBank: BC000798
• Symbole du gène: ARPC5L
• Identification du gène (NCBI): 81873
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARPC5L, also known as ARC16-2, belongs to the ARPC5 family. ARPC5L is involved in Arp2/3 complex-mediated actin nucleation and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARPC5L-containing Arp2/3 complexes are required for the peripheral positioning of nuclei in myofibers (PMID: 28892082). The molecular mass of ARPC5L is 17-20 kDa.

Publications

Species	Application	Title
human	WB,IF	Pigment Cell Melanoma Res; YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5); Authors - Jason W. Lui