Anticorps Polyclonal de lapin anti-ASCC2

ASCC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11529-1-AP

Synonymes

ASC 1 complex subunit p100, ASC1p100, ASCC2, Trip4 complex subunit p100

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Galerie de données de validation

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  WB analysis using 11529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11529-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 11529-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 11529-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11529-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11529-1-AP

  IP Result of anti-ASCC2 (IP:11529-1-AP, 4ug; Detection:11529-1-AP 1:1000) with HeLa cells lysate 2640ug.

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  IHC staining of human colon cancer using 11529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11529-1-AP (ASCC2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 11529-1-AP

  Immunofluorescent analysis of HepG2 cells, using ASCC2 antibody 11529-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 11529-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug ASCC2 antibody (11529-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu de côlon de souris, tissu de côlon humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

11529-1-AP cible ASCC2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASCC2 Protéine recombinante Ag2100
• Nom complet: activating signal cointegrator 1 complex subunit 2
• Masse moléculaire calculée: 757 aa, 86 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC025368
• Symbole du gène: ASCC2
• Identification du gène (NCBI): 84164
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex; Authors - Junqiao Jia
	WB	Nat Commun; The interaction of DNA repair factors ASCC2 and ASCC3 is affected by somatic cancer mutations.; Authors - Junqiao Jia
mouse	WB	Chin Med; Du Huo Ji Sheng Tang inhibits Notch1 signaling and subsequent NLRP3 activation to alleviate cartilage degradation in KOA mice; Authors - Wen-Jin Chen