Anticorps Polyclonal de lapin anti-ASGR1

• Phare
• Validé par KD/KO

ASGR1 Polyclonal Antibody for IF-P, FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF-P, FC

Conjugaison

CoraLite®555 Fluorescent Dye

Avis (2)

N° de cat : CL555-11739

Synonymes

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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Galerie de données de validation

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  IF Staining of rat liver using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

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  IF Staining of rat liver using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

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  IF Staining of human liver cancer using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

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  IF Staining of human liver cancer using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

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  FC experiment of HepG2 using CL555-11739

  1X10^6 HepG2 cells were surface stained with 0.4 ug CoraLite®555 Anti-Human ASGR1 (CL555-11739) (red), or 0.4 ug Control Antibody. Cells were not fixed .

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Applications testées

• Résultats positifs en IF-P: tissu hépatique de rat, tissu de cancer du foie humain
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-11739 cible ASGR1 dans les applications de IF-P, FC et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASGR1 Protéine recombinante Ag2310
• Nom complet: asialoglycoprotein receptor 1
• Masse moléculaire calculée: 291 aa, 33 kDa
• Numéro d’acquisition GenBank: BC032130
• Symbole du gène: ASGR1
• Identification du gène (NCBI): 432
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rupa (Verified Customer) (04-26-2024)

Worked very well with immunofluorescence and flowcytometry

• Applications: Immunohistochemistry, Flow Cytometry
• Primary Antibody Dilution: 1-100
• Cell Tissue Type: mouse liver

FH

Parmita (Verified Customer) (04-23-2024)

worked fine for us. signal was sharp and specific detection observed

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse liver tissue

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