Anticorps Polyclonal de lapin anti-ASPM

• Phare
• Validé par KD/KO

ASPM Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 26223-1-AP

Synonymes

ASP, Asp homolog, ASPM, Calmbp1, MCPH5

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Galerie de données de validation

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  WB analysis using 26223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26223-1-AP (ASPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26223-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26223-1-AP (ASPM antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with alpha tubulin (66031-1-Ig, red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules K-562
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de gliome humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

26223-1-AP cible ASPM dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASPM Protéine recombinante Ag24166
• Nom complet: asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
• Masse moléculaire calculée: 410 kDa or 218 kDa
• Poids moléculaire observé: 218 kDa
• Numéro d’acquisition GenBank: BC034607
• Symbole du gène: ASPM
• Identification du gène (NCBI): 259266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Aging (Albany NY); ASPM promotes glioblastoma growth by regulating G1 restriction point progression and Wnt-β-catenin signaling.; Authors - Xin Chen ; KD Validated
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	Front Oncol; Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis.; Authors - Yingji Chen
human	WB	DNA Cell Biol; ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth.; Authors - Qian Yang
human	WB,IHC	Int J Endocrinol; ASPM Promotes the Progression of Anaplastic Thyroid Carcinomas by Regulating the Wnt/β-Catenin Signaling Pathway.; Authors - Liang Jiang ; KD Validated
human	WB,IHC	Gland Surg; Abnormal spindle-like microcephaly-associated protein promotes proliferation by regulating cell cycle in epithelial ovarian cancer.; Authors - Yiguo Wu ; KD Validated