Anticorps Polyclonal de lapin anti-ASRGL1

ASRGL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 11400-1-AP

Synonymes

ALP, ALP1, Asparaginase-like protein 1, ASRGL 1, Beta-aspartyl-peptidase

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Galerie de données de validation

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  WB analysis of HeLa using 11400-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11400-1-AP (ASRGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11400-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11400-1-AP (ASRGL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11400-1-AP

  IP result of anti-ASRGL1 (IP:11400-1-AP, 4ug; Detection:11400-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human gliomas using 11400-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11400-1-AP (ASRGL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11400-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASRGL1 antibody (11400-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

11400-1-AP cible ASRGL1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASRGL1 Protéine recombinante Ag1955
• Nom complet: asparaginase like 1
• Masse moléculaire calculée: 308 aa, 32 kDa
• Poids moléculaire observé: 32-40 kDa, 20-25 kDa, 15-19 kDa
• Numéro d’acquisition GenBank: BC021295
• Symbole du gène: ASRGL1
• Identification du gène (NCBI): 80150
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Asparaginase-like protein 1 (ASRGL1) is also named as ALP, CRASH and belongs to the Ntn-hydrolase family. ASRGL1 has both L-asparaginase and beta-aspartyl peptidase activity. It may be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. ASRGL1 has 2 isoforms produced by alternative splicing with the MW of 32 kDa and 19 kDa. Two lower molecular weight bands (23 and 15 kDa) can be detected corresponding to the processed α and β subunits (PMID: 27106100).

Publications

Species	Application	Title
human		Cell Metab; As Extracellular Glutamine Levels Decline, Asparagine Becomes an Essential Amino Acid.; Authors - Natalya N Pavlova
mouse	WB	Nat Chem Biol; PMID: 28671681; Authors - Nunzio Iraci
mouse	IF	Hum Mol Genet; A missense mutation in the ASRGL1 gene is involved in causing autosomal recessive retinal degeneration.; Authors - Pooja Biswas
cat	IHC	Front Cell Dev Biol; Oocyte Meiotic Competence in the Domestic Cat Model: Novel Roles for Nuclear Proteins BRD2 and NPM1.; Authors - Daniela R Chavez
human	WB	Front Oncol; ASRGL1 Correlates With Immune Cell Infiltration in Hepatocellular Carcinoma and Can Serve as a Prognostic Biomarker.; Authors - Cailin Xue
mouse	WB	Inflammation; Ghrelin Fights Against Titanium Particle-Induced Inflammatory Osteolysis Through Activation of β-Catenin Signaling Pathway.; Authors - Ruize Qu

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