Anticorps Monoclonal anti-ASS1

• Phare
• Validé par KD/KO

ASS1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, IF

Conjugaison

Non conjugué

Publications

4

CloneNo.

1D1A11

N° de cat : 66036-1-Ig

Synonymes

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1

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Galerie de données de validation

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  WB analysis of pig liver using 66036-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66036-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66036-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 66036-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human liver using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human testis using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66036-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using ASS1 antibody 66036-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de porc, cellules HeLa, cellules L02, tissu hépatique humain
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du foie humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

66036-1-Ig cible ASS1 dans les applications de WB, IHC, IF et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASS1 Protéine recombinante Ag9314
• Nom complet: argininosuccinate synthetase 1
• Masse moléculaire calculée: 412 aa, 47 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC009243
• Symbole du gène: ASS1
• Identification du gène (NCBI): 445
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Publications

Species	Application	Title
human	WB	FASEB J; Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.; Authors - Chung-Ying Tsai ; KD Validated
human	WB	Mol Pharmacol; MicroRNA-1291-5p Sensitizes Pancreatic Carcinoma Cells to Arginine Deprivation and Chemotherapy through the Regulation of Arginolysis and Glycolysis.; Authors - Mei-Juan Tu
human	WB	BMC Cancer; PMID: 25240403; Authors - Jiangbo Liu
human	WB	BMC Cancer; Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy.; Authors - Qingyuan Feng