Anticorps Recombinant de lapin anti-ATAD1

ATAD1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241449D12

N° de cat : 84194-6-RR

Synonymes

241449D12, AFDC1, ATAD 1, ATPase family AAA domain-containing protein 1, EC:7.4.2.-

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Galerie de données de validation

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  WB analysis using 84194-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84194-6-RR (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84194-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATAD1 antibody (84194-6-RR, Clone: 241449D12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A431 using 84194-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug ATAD1 Recombinant antibody (84194-6-RR, Clone:241449D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84194-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84194-6-RR against Human ATAD1 were performed. The affinity constant is 0.573 nM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84194-6-RR cible ATAD1 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATAD1 Protéine recombinante Ag10343
• Nom complet: ATPase family, AAA domain containing 1
• Masse moléculaire calculée: 361 aa, 41 kDa
• Poids moléculaire observé: 32-37 kDa
• Numéro d’acquisition GenBank: BC010868
• Symbole du gène: ATAD1
• Identification du gène (NCBI): 84896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.

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