Anticorps Polyclonal de lapin anti-ATF2-Specific

• Phare
• Validé par KD/KO

ATF2-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 14834-1-AP

Synonymes

ATF2, ATF2-Specific, CRE BP1, CREB 2, CREB2, CREBP1, HB16, TREB7

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Galerie de données de validation

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  WB analysis of HeLa using 14834-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14834-1-AP (ATF2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 14834-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 14834-1-AP (ATF2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 14834-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 14834-1-AP (ATF2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14834-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14834-1-AP (ATF2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14834-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14834-1-AP (ATF2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14834-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14834-1-AP (ATF2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14834-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14834-1-AP (ATF2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C2C12, cellules LNCaP
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

14834-1-AP cible ATF2-Specific dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: activating transcription factor 2
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC026175
• Symbole du gène: ATF2
• Identification du gène (NCBI): 1386
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human ATF2. The calculated molecular weight of ATF2 is 55 kDa, but the modified ATF2 protein is about 70 kDa (PMID: 18269766 ).

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng
mouse	WB	Cell Rep; Cold-Inducible SIRT6 Regulates Thermogenesis of Brown and Beige Fat.; Authors - Lu Yao
human	WB	J Biol Chem; MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1.; Authors - Fanpeng Zhao
human	WB	Artif Cells Nanomed Biotechnol; Molecular mechanism of miR-204 regulates proliferation, apoptosis and autophagy of cervical cancer cells by targeting ATF2.; Authors - Nan Li ; KD Validated

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