Anticorps Polyclonal de lapin anti-ATG16L1

ATG16L1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (5)

N° de cat : 19812-1-AP

Synonymes

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, IBD10, WDR30

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Galerie de données de validation

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  WB analysis of mouse spleen using 19812-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 19812-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19812-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19812-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19812-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 19812-1-AP

  IP Result of anti-ATG16L1 (IP:19812-1-AP, 4ug; Detection:19812-1-AP 1:300) with MCF-7 cells lysate 2000ug.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules HEK-293T, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

19812-1-AP cible ATG16L1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG16L1 Protéine recombinante Ag13844
• Nom complet: ATG16 autophagy related 16-like 1 (S. cerevisiae)
• Masse moléculaire calculée: 607 aa, 68 kDa
• Poids moléculaire observé: 63-71 kDa
• Numéro d’acquisition GenBank: BC000061
• Symbole du gène: ATG16L1
• Identification du gène (NCBI): 55054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

Publications

Species	Application	Title
human	IF	Autophagy; ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1; Authors - Weihong Xie
human	IF	Nat Commun; Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.; Authors - Shuang-Zhou Peng
human	WB	Autophagy; AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.; Authors - Ashwinkumar Subramenium Ganapathy
human	WB	Curr Biol; Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.; Authors - Anna Ulbricht
human	WB,IHC	Cancer Lett; Let-7i-5p promotes a malignant phenotype in nasopharyngeal carcinoma via inhibiting tumor-suppressive autophagy.; Authors - Bo You
human	WB	Clin Transl Immunology; Circular RNA TRAPPC6B inhibits intracellular Mycobacterium tuberculosis growth while inducing autophagy in macrophages by targeting microRNA-874-3p.; Authors - Hou-Long Luo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (07-31-2023)

Used for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

X (Verified Customer) (01-18-2021)

It is OK to use it in WB with human postmortem brain lysate. There is band with MW as expected, although there is other unidentified bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human postmortem brain lysate

ATG16L1 Antibody Western Blot validation (1:1000 dilution) in human postmortem brain lysate (Cat no:19812-1-AP)