Anticorps Polyclonal de lapin anti-ATG3

ATG3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(26)

N° de cat : 11262-2-AP

Synonymes

Autophagy-related protein 3, ATG 3, APG3-like, APG3L, APG3 LIKE

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Galerie de données de validation

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  WB analysis using 11262-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11262-2-AP (ATG3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 11262-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11262-2-AP (ATG3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11262-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11262-2-AP (ATG3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 11262-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATG3 antibody (11262-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules Jurkat, cellules K-562, cellules PC-12, cellules THP-1
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 24 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11262-2-AP cible ATG3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG3 Protéine recombinante Ag1785
• Nom complet: ATG3 autophagy related 3 homolog (S. cerevisiae)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC024221
• Symbole du gène: ATG3
• Identification du gène (NCBI): 64422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Autophagy is a lysosome-dependent conserved catabolic process in response to various stresses, such as oxidative stress and ischemia, for the degradation and recycling of aged or dysfunctional intracellular components and damaged organelles. A large group of autophagy-related genes (ATGs) have been identifed as essential drivers in diferent stages of autophagy. Autophagy-related 3 (ATG3), one of the autophagyrelated gene family members, serves as an E2-like enzyme contributing to the conjugation of microtubule-associated protein light chain 3 (LC3) to lipid phosphotidylethanolamine (PE). ATG3 plays a signifcant role in the regulation of autophagy, viability, and death.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.; Authors - Bo Wu
pig	WB	Chemosphere; Genome-wide transcriptional profiling and functional analysis reveal miR-330-MAPK15 axis involving in cellular responses to deoxynivalenol exposure.; Authors - Haifei Wang
mouse	WB	Front Cell Dev Biol; Aquaporin-8 transports hydrogen peroxide to regulate granulosa cell autophagy; Authors - Binbin Huang
mouse	WB	Front Immunol; Toxoplasma gondii CDPK3 Controls the Intracellular Proliferation of Parasites in Macrophages.; Authors - Minmin Wu
human	WB	Oncotarget; Upregulation of the lncRNA Meg3 induces autophagy to inhibit tumorigenesis and progression of epithelial ovarian carcinoma by regulating activity of ATG3.; Authors - Yin-Ling Xiu
mouse	WB	Ecotoxicol Environ Saf; Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.; Authors - Yifan Hong

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