Anticorps Polyclonal de lapin anti-ATG4D

• Phare
• Validé par KD/KO

ATG4D Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 16924-1-AP

Synonymes

APG4 D, APG4D, ATG, AUTL4, AUT-like 4 cysteine endopeptidase

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Galerie de données de validation

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  WB analysis of human brain using 16924-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16924-1-AP (ATG4D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16924-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16924-1-AP (ATG4D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 16924-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATG4D antibody (16924-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below

Informations sur le produit

16924-1-AP cible ATG4D dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG4D Protéine recombinante Ag10307
• Nom complet: ATG4 autophagy related 4 homolog D (S. cerevisiae)
• Masse moléculaire calculée: 474 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC068992
• Symbole du gène: ATG4D
• Identification du gène (NCBI): 84971
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.

Publications

Species	Application	Title
human	WB	Cancer Cell; MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma.; Authors - Tianzhi Huang
human	WB	Mol Cell; Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine.; Authors - Joanne Durgan ; KO Validated
human,mouse	WB	ACS Sens; Air-Liquid Interface Microfluidic Monitoring Sensor Platform for Studying Autophagy Regulation after PM2.5 Exposure; Authors - Lulu Zheng
human	WB	Cell Rep; Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation; Authors - Rachel Ulferts ; KD Validated
human	WB	Mol Ther Nucleic Acids; lncRNA RMST Suppressed GBM Cell Mitophagy through Enhancing FUS SUMOylation.; Authors - Changhong Liu
human	WB	Autophagy; Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.; Authors - Alexander Agrotis ; KD Validated

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