Anticorps Polyclonal de lapin anti-ATG5

• Phare
• Validé par KD/KO

ATG5 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

254

Avis (5)

N° de cat : 10181-2-AP

Synonymes

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

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Galerie de données de validation

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  WB analysis using 10181-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10181-2-AP

  WB result of ATG5 antibody (10181-2-AP; 1:2000; room temperature for 1.5 hours) with wild-type and ATG5 knockout HeLa cells.

• 

  WB analysis using 10181-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10181-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10181-2-AP

  IP result of anti-ATG5(IP:10181-2-AP, 4ug; Detection:10181-2-AP 1:3000) with HeLa cells lysate 1760 ug.

• 

  IHC staining of human colon cancer using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 10181-2-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ATG5 (10181-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 39 publications below
• WB: See 231 publications below
• IHC: See 22 publications below
• IF: See 22 publications below
• FC: See 2 publications below

Informations sur le produit

10181-2-AP cible ATG5 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, poulet, singe, souris, mérou
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG5 Protéine recombinante Ag0214
• Nom complet: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa, 40-45 kDa, 50-55 kDa
• Numéro d’acquisition GenBank: BC002699
• Symbole du gène: ATG5
• Identification du gène (NCBI): 9474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
human	WB	Cell Res; Degradation of lipid droplets by chimeric autophagy-tethering compounds.; Authors - Yuhua Fu ; KO Validated
human	WB	Mol Cell; Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.; Authors - Lu Yubing Y ; KD Validated
human	WB	Adv Sci (Weinh); SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle; Authors - Xiaowei She
human		Autophagy; Autophagy mediates the clearance of oligodendroglial SNCA/alpha-synuclein and TPPP/p25A in multiple system atrophy models; Authors - Panagiota Mavroeidi
mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (08-09-2023)

Used for Caco2 cells and mice colon tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Kamal (Verified Customer) (06-29-2023)

Works well with TBST. Atg5-12 complex (56 kDa) and Atg5 (33kDa) bands came out nice and clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Liver tissue

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Benjamin (Verified Customer) (01-07-2020)

Works well in western blot in 3% milk TBS-T

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK239 Mammalian Cell Lysate

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