Anticorps Monoclonal anti-ATG5

• Phare
• Validé par KD/KO

ATG5 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

3G6E6

N° de cat : 60061-1-Ig

Synonymes

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

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Galerie de données de validation

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  WB analysis of HeLa using 60061-1-Ig

  WB result of ATG5 antibody (60061-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HeLa cells.

• 

  WB analysis of K-562 using 60061-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60061-1-Ig (ATG5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60061-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60061-1-Ig (ATG5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 60061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60061-1-Ig (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60061-1-Ig (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60061-1-Ig (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60061-1-Ig (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60061-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60061-1-Ig(ATG5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60061-1-Ig cible ATG5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATG5 Protéine recombinante Ag0214
• Nom complet: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC002699
• Symbole du gène: ATG5
• Identification du gène (NCBI): 9474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection.

Publications

Species	Application	Title
mouse	WB	Autophagy; Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice.; Authors - Qian Huang
mouse	IF	Autophagy; The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.; Authors - Andreas Struchtrup
human,mouse	WB,IHC	Cancer Cell Int; PCK1 activates oncogenic autophagy via down-regulation Serine phosphorylation of UBAP2L and antagonizes colorectal cancer growth; Authors - Xiangyan Zhang
human	WB	Cell Biosci; A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.; Authors - Juan Yin
human	WB	Mol Med Rep; Autophagy is essential for flavopiridol‑induced cytotoxicity against MCF‑7 breast cancer cells.; Authors - Shuo Wang ; KD Validated
mouse	WB	Cells Dev; Enhanced autophagy suppresses inflammation-mediated bone loss through ROCK1 signaling in bone marrow mesenchymal stem cells.; Authors - Jingjing Zheng

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