Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66744-1-Ig targets ATG5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, bovine |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16685 Product name: Recombinant human ATG5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 28-275 aa of BC002699 Sequence: TEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD Predict reactive species |
| Full Name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 50-55 and 40-45 kDa |
| GenBank Accession Number | BC002699 |
| Gene Symbol | ATG5 |
| Gene ID (NCBI) | 9474 |
| RRID | AB_2882092 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H1Y0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ATG5 antibody 66744-1-Ig | Download protocol |
| WB protocol for ATG5 antibody 66744-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATG5 Monoclonal antibody (Cat# 66744-1-Ig) has accumulated 68 citations across journals including Autophagy, Nature Communications, Gastroenterology, Cell Death & Disease, Cancer Letters, Journal of Nanobiotechnology, Redox Biology, Developmental Cell, and Frontiers in Pharmacology. Associated research fields encompass autophagy, ferroptosis, cancer biology, neurology, renal and liver disease, inflammation, cardiovascular pathology, toxicology, and metabolic disorders.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. | ||
Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells | ||
Cell Mol Immunol Dynamic regulation of TBK1 lactylation shapes antiviral immune responses. | ||
Autophagy Advancing targeted protein degradation: pLIRTAC's role in glioma and CAR-T cell therapy.
| ||
Autophagy Regorafenib enhances anti-PDCD1/PD-1 therapeutic efficacy in colorectal cancer by promoting SQSTM1/p62-mediated CD274/PD-L1 degradation. | ||
Autophagy Cerebral FURIN deficiency impairs astrocytic lipophagy through ITGAV maturation. |
Reviews
What customers say
The single review for this antibody is highly positive, rated 5 stars. The reviewer used it for Western Blot at a 1:500 dilution in 1% skim milk in TBST on primary mouse microglia. They reported clear detection of ATG5 and the ATG5-ATG12 complex with almost no background, indicating strong specificity and low non-specific binding. Overall, the feedback highlights reliable performance and clean results in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marina (Verified Customer) (11-22-2021) | ATG 5 and the complex ATG5-ATG12 are visible, almost no background.
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