Anticorps Polyclonal de lapin anti-ATG7

• Phare
• Validé par KD/KO

ATG7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (5)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

103

Avis (3)

N° de cat : 10088-2-AP

Synonymes

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, GSA7, hAGP7

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Galerie de données de validation

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  WB analysis using 10088-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10088-2-AP (ATG7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10088-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10088-2-AP (ATG7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-1080 using 10088-2-AP

  IP result of anti-ATG7(IP:10088-2-AP, 4ug; Detection:10088-2-AP 1:500) with HT-1080 cells lysate 3600 ug.

• 

  IP experiment of NIH/3T3 using 10088-2-AP

  IP result of anti-ATG7(IP:10088-2-AP, 4ug; Detection:10088-2-AP 1:500) with NIH/3T3 cells lysate 1600 ug.

• 

  IHC staining of human cervical cancer using 10088-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10088-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10088-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10088-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10088-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG7 antibody (10088-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules NIH/3T3, cellules THP-1, cellules U2OS, tissu hépatique de souris
• Résultats positifs en IP: cellules HT-1080, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 17 publications below
• WB: See 93 publications below
• IHC: See 14 publications below
• IF: See 7 publications below

Informations sur le produit

10088-2-AP cible ATG7 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Chèvre, Humain, porc, poulet, souris, crevette
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG7 Protéine recombinante Ag0142
• Nom complet: ATG7 autophagy related 7 homolog (S. cerevisiae)
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 68-78 kDa
• Numéro d’acquisition GenBank: BC000091
• Symbole du gène: ATG7
• Identification du gène (NCBI): 10533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
human	WB	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang ; KD Validated
human	WB	Cell Death Differ; STING directly activates autophagy to tune the innate immune response.; Authors - Dong Liu
mouse	WB, IHC	Autophagy; Autophagy regulates functional differentiation of mammary epithelial cells.; Authors - Jessica Elswood
rat	WB	Autophagy; Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis.; Authors - Sheng Zhang
human,mouse	WB	Autophagy; AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.; Authors - Ashwinkumar Subramenium Ganapathy ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (08-09-2023)

Used for Caco2 cells and mice colon tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Kamal (Verified Customer) (06-29-2023)

Atg7 band showed up better than from other companies.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Liver tissue

ATG7 Antibody Western Blot validation (1:2000 dilution) in Mouse Liver tissue (Cat no:10088-2-AP)

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

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