Anticorps Monoclonal anti-ATG7

• Phare
• Validé par KD/KO

ATG7 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

25

CloneNo.

2B5A10

N° de cat : 67341-1-Ig

Synonymes

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7

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Galerie de données de validation

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  WB analysis using 67341-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 67341-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67341-1-Ig

  WB result of ATG7 antibody (67341-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells.

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  WB analysis of 4T1 using 67341-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spleen using 67341-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 67341-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67341-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67341-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67341-1-Ig (ATG7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67341-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATG7 (67341-1-Ig, Clone:2B5A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 67341-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATG7 (67341-1-Ig, Clone:2B5A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules THP-1, tissu splénique de porc
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

67341-1-Ig cible ATG7 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATG7 Protéine recombinante Ag29172
• Nom complet: ATG7 autophagy related 7 homolog (S. cerevisiae)
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 68-78 kDa
• Numéro d’acquisition GenBank: BC000091
• Symbole du gène: ATG7
• Identification du gène (NCBI): 10533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Publications

Species	Application	Title
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	Cancers (Basel); Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators.; Authors - David P Dannheisig
mouse	WB	J Cell Physiol; Cold exposure-induced endoplasmic reticulum stress regulates autophagy through the SIRT2/FoxO1 signaling pathway; Authors - Jingru Guo
mouse	WB	Stem Cell Res Ther; Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.; Authors - Jie Zhou
mouse	WB	Phytomedicine; Trillin inhibits myoblast differentiation via increasing autophagy.; Authors - Weilong Dai
mouse	WB	Front Pharmacol; Cardamonin Reduces Acetaminophen-Induced Acute Liver Injury in Mice via Activating Autophagy and NFE2L2 Signaling.; Authors - Qiushi Xu