Anticorps Polyclonal de lapin anti-GABARAPL1

• Phare
• Validé par KD/KO

GABARAPL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

57

Avis (1)

N° de cat : 11010-1-AP

Synonymes

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GEC 1, GEC1

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Galerie de données de validation

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  WB analysis using 11010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11010-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11010-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11010-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11010-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11010-1-AP

  IP Result of anti-GABARAPL1-Specific (IP:11010-1-AP, 3ug; Detection:11010-1-AP 1:500) with mouse liver tissue lysate 5000ug.

• 

  IHC staining of mouse brain using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11010-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11010-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using GABARAPL1-Specific antibody (11010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 11010-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using GABARAPL1-Specific antibody (11010-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 11010-1-AP

  Immunofluorescent analysis of Chloroquine treated HepG2 cells using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 11010-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug GABARAPL1-Specific antibody (11010-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2, tissu cardiaque humain, tissu cérébral de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de tumeur ovarienne humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa traitées à la chloroquine, cellules HepG2 traitées à la chloroquine
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 46 publications below
• IHC: See 6 publications below
• IF: See 19 publications below
• IP: See 3 publications below

Informations sur le produit

11010-1-AP cible GABARAPL1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris, crevette
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GABARAPL1 Protéine recombinante Ag1473
• Nom complet: GABA(A) receptor-associated protein like 1
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC009309
• Symbole du gène: GABARAPL1
• Identification du gène (NCBI): 23710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine. The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.

Publications

Species	Application	Title
human	WB	Circulation; Markers of autophagy are downregulated in failing human heart after mechanical unloading.; Authors - Kassiotis Christos C
human	WB,IF,IP	J Extracell Vesicles; Secretion of pro-angiogenic extracellular vesicles during hypoxia is dependent on the autophagy-related protein GABARAPL1.; Authors - Tom G Keulers
mouse	WB	Cell Metab; FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells.; Authors - Zhao Jinghui J
mouse	WB	Autophagy; Analysis of ATG4C function in vivo; Authors - Isaac Tamargo-Gómez
mouse	WB,IF	J Clin Invest; Autophagy is essential for mouse sense of balance.; Authors - Mari?o Guillermo G
human	WB	Autophagy; GABARAPL1 antibodies: target one protein, get one free!; Authors - Le Grand Jaclyn Nicole JN

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (04-06-2020)

I could not detect GABARAP L1 in HEK293T and HCT116 cells. It is possible that the expression level of GABARAP L1 in these two cell lines are extremely low. In proteomics analysis of the HEK293T whole cell lysates, however, we could detect GABARAP L1.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T and HCT116

GABARAPL1 Antibody Western Blot validation (1:1000 dilution) in HEK293T and HCT116 (Cat no:11010-1-AP)

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