Anticorps Polyclonal de lapin anti-ATGL

• Phare
• Validé par KD/KO

ATGL Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(89)

Avis (2)

N° de cat : 55190-1-AP

Synonymes

Adipose triglyceride lipase, PNPLA2, Calcium-independent phospholipase A2-zeta, Desnutrin, EC:3.1.1.3

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Galerie de données de validation

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  WB analysis of 3T3-L1 using 55190-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55190-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55190-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55190-1-AP

  WB result of ATGL antibody (55190-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATGL transfected A431 cells.

• 

  WB analysis using 55190-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 55190-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 55190-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55190-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 55190-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55190-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55190-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 55190-1-AP

  IP result of anti-ATGL (IP:55190-1-AP, 4ug; Detection:55190-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules 3T3-L1, cellules A431, cellules HeLa, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu hépatique de rat, tissu hépatique de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 84 publications below
• IHC: See 5 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

55190-1-AP cible ATGL dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poisson-zèbre, porc, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: patatin-like phospholipase domain containing 2
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: NM_020376
• Symbole du gène: ATGL
• Identification du gène (NCBI): 57104
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.

Publications

Species	Application	Title
mouse	WB	J Pineal Res; Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin.; Authors - Zhenhui Li
human		Hepatology; PMID: 29729186; Authors - Huixia Zhang
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li
mouse	WB	Food Chem; Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.; Authors - Shanshan Hu
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Lipodystrophic gene Agpat2 deficiency aggravates hyperlipidemia and atherosclerosis in Ldlr-/- mice; Authors - Kenan Peng
rat,human	WB	Front Pharmacol; Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism; Authors - Tian Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Li (Verified Customer) (07-12-2019)

We have tried a couple different ATGL antibodies from other vendors, but no one is working as good as we want. So we tried this one, it works great.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse muscle

FH

Shantel (Verified Customer) (05-02-2019)

Works well in Western Blot. I used mouse liver tissue, 40ug/ul protein per lane at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver

ATGL Antibody Western Blot, validation (1:1000 dilution) in mouse liver (Cat no:55190-1-AP)

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