Anticorps Polyclonal de lapin anti-ATOH1

ATOH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), chIP, ELISA

Conjugaison

Non conjugué

Publications(50)

Avis (7)

N° de cat : 21215-1-AP

Synonymes

ATH1, bHLHa14, Class A basic helix-loop-helix protein 14, HATH1, Helix-loop-helix protein hATH-1

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Galerie de données de validation

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  WB analysis using 21215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21215-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21215-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21215-1-AP (ATOH1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21215-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21215-1-AP (ATOH1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATOH1 antibody (21215-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATOH1 antibody (21215-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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  IF Staining of HepG2 using 21215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21215-1-AP (ATOH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 21215-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ATOH1 (21215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules C6, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 23 publications below
• IHC: See 4 publications below
• IF: See 22 publications below
• ChIP: See 1 publications below

Informations sur le produit

21215-1-AP cible ATOH1 dans les applications de WB, IHC, IF/ICC, FC (Intra), chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATOH1 Protéine recombinante Ag15615
• Nom complet: atonal homolog 1 (Drosophila)
• Masse moléculaire calculée: 354 aa, 38 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC069594
• Symbole du gène: ATOH1
• Identification du gène (NCBI): 474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATOH1, also named as Protein atonal homolog 1 or BHLHA14, is a 364 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. ATOH1,as a transcriptional regulator in the nucleus activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. ATOH1 plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.

Publications

Species	Application	Title
human	IF	Cell Res; Decoding the spatiotemporal regulation of transcription factors during human spinal cord development; Authors - Yingchao Shi
human	IF	Immunity; IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.; Authors - Xun Lin
human	WB	Nat Cell Biol; Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.; Authors - Donglim Esther Park
mouse	IHC	J Clin Invest; Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea.; Authors - Patrick J Atkinson
	IF	Dev Cell; Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling; Authors - Stefanie Lehr
mouse	IF	Proc Natl Acad Sci U S A; Sufu- and Spop-mediated regulation of Gli2 is essential for the control of mammalian cochlear hair cell differentiation; Authors - Tianli Qin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nicole (Verified Customer) (08-08-2024)

Works really well both in Western Blot and Immunofluorescence

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500

FH

Yin (Verified Customer) (12-20-2021)

It did not work.

• Applications: Immunofluorescence
• Cell Tissue Type: Cerebella granule cell

FH

Stephen (Verified Customer) (11-04-2020)

ATOH1 antibody was tested on embryonic chicken tissue. Staining showed nuclear localisation in inner ear vestibular crista in sox2 positive prosensory region of 6 day old embryo

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Embryonic chicken inner ear tissue

ATOH1 Antibody Immunofluorescence validation (1:300 dilution) in Embryonic chicken inner ear tissue (Cat no:21215-1-AP)

FH

Roland (Verified Customer) (09-17-2020)

We tested several ATOH1 antibodies and this is the only one which turned out to be specific as confirmed bei knockdown experiments.

• Applications: Western Blot
• Cell Tissue Type: human cancer cells

FH

Biren (Verified Customer) (08-31-2020)

I used human GBM stem cells as a negative control to validate the specificity of this antibody. The cells used were confirmed by RNAseq to not express ATOH1, yet all cells were positively stained at the previously used concentration of 1/500, indicating that this antibody is not specific.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: human glioblastoma stem cells

ATOH1 Antibody Immunofluorescence validation (1/500 dilution) in human glioblastoma stem cells (Cat no:21215-1-AP)

FH

Erithelgi (Verified Customer) (09-25-2019)

The antibody detects a band of the expected size, in cells that induce GFP-ATOH1 under a DOX promoter. No bands detected in non-induced cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: medulloblastoma

FH

Nicole (Verified Customer) (07-23-2019)

Used to stain cultured stem cells for differentiation markers.

• Applications: Immunohistochemistry, Immunofluorescence,
• Cell Tissue Type: Stem Cell culture

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