Anticorps Polyclonal de lapin anti-ATP2A1

ATP2A1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 22361-1-AP

Synonymes

SR Ca(2+)-ATPase 1, SERCA1, EC:7.2.2.10, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, ATP2A 1

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Galerie de données de validation

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  WB analysis using 22361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22361-1-AP (ATP2A1 antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 22361-1-AP

  IP result of anti-ATP2A1 (IP:22361-1-AP, 5ug; Detection:22361-1-AP 1:700) with mouse skeletal muscle tissue lysate 2160ug.

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  IHC staining of human skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22361-1-AP (ATP2A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22361-1-AP (ATP2A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique de rat
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cardiaque humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

22361-1-AP cible ATP2A1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP2A1 Protéine recombinante Ag17944
• Nom complet: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Masse moléculaire calculée: 1001 aa, 110 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC037354
• Symbole du gène: ATP2A1
• Identification du gène (NCBI): 487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP2A1 also known as SERCA1, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen and is involved in muscular excitation and contraction. Mutations in ATP2A1 cause some autosomal recessive forms of Brody disease(PMID: 23911890, 10914677).

Publications

Species	Application	Title
mouse	IHC,IF	Int J Mol Sci; The Effects of Aging on Sarcoplasmic Reticulum-Related Factors in the Skeletal Muscle of Mice; Authors - Yuji Kanazawa
mouse	WB	J Endocrinol; ChREBP-β regulates thermogenesis in brown adipose tissue.; Authors - Chunchun Wei
chicken	WB	Metallomics; Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken.; Authors - Jie Yang
mouse	WB	Physiol Behav; Exposure to melamine cyanuric acid in adult mice caused motor activity and skeletal muscle energy metabolism disorder; Authors - Sijie Wang
mouse	WB	J Muscle Res Cell Motil; Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.; Authors - Nagakatsu Harada
	WB	Mol Ther Nucleic Acids; lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.; Authors - Jie Yang

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