Anticorps Polyclonal de lapin anti-ATP5I

ATP5I Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 16483-1-AP

Synonymes

ATP5I, ATP5K, ATP5ME, ATPase subunit e

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Galerie de données de validation

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  WB analysis using 16483-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16483-1-AP (ATP5I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16483-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16483-1-AP (ATP5I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16483-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16483-1-AP (ATP5I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16483-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5I antibody (16483-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

16483-1-AP cible ATP5I dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP5I Protéine recombinante Ag9605
• Nom complet: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E
• Masse moléculaire calculée: 69 aa, 8 kDa
• Poids moléculaire observé: 8 kDa
• Numéro d’acquisition GenBank: BC003679
• Symbole du gène: ATP5I
• Identification du gène (NCBI): 521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP5I(ATP synthase subunit e) is also named as ATP5K and belongs to the ATPase e subunit family. The ATP5I gene encodes the e subunit of the mitochondrial ATP synthase Fo complex. Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. Antisense ATP5I in a human HCC cell line inhibited cell growth suggesting that ATP5I acts through the MAP kinase pathway(PMID:11939412).

Publications

Species	Application	Title
human		EBioMedicine; Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases.; Authors - Tetsuro Matsuhashi
mouse	WB	Front Aging Neurosci; Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.; Authors - Keqiang He
chicken	WB	Genet Sel Evol; The mRNA-lncRNA landscape of multiple tissues uncovers key regulators and molecular pathways that underlie heterosis for feed intake and efficiency in laying chickens; Authors - Jingwei Yuan
	WB	J Proteomics; Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.; Authors - Víctor Llombart
human	IF	Biology (Basel); Mitochondrial Translation Occurs Preferentially in the Peri-Nuclear Mitochondrial Network of Cultured Human Cells.; Authors - Christin A Albus
	WB	Life Sci Alliance; MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes.; Authors - Ruchika Anand

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yan (Verified Customer) (01-28-2020)

It worked well when I performed western blot in embryonic tissue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse embryonic tissue