Anticorps Polyclonal de lapin anti-ATP6V1A

• Phare
• Validé par KD/KO

ATP6V1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(44)

Avis (1)

N° de cat : 17115-1-AP

Synonymes

V-ATPase subunit A, V-ATPase 69 kDa subunit, VA68, EC:7.1.2.2, ATP6V1A1

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Galerie de données de validation

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  WB analysis using 17115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17115-1-AP

  WB result of ATP6V1A antibody (17115-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells.

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  WB analysis of mouse kidney using 17115-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17115-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17115-1-AP

  IP result of anti-ATP6V1A (IP:17115-1-AP, 4ug; Detection:17115-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human pancreas using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17115-1-AP (ATP6V1A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17115-1-AP (ATP6V1A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human thyroid cancer using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17115-1-AP (ATP6V1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17115-1-AP (ATP6V1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17115-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP6V1A antibody (17115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of NIH/3T3 using 17115-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ATP6V1A antibody (17115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules Daudi, cellules HeLa, cellules HepG2, cellules K-562, cellules MG U-87, tissu placentaire humain, tissu rénal de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 32 publications below
• IHC: See 2 publications below
• IF: See 14 publications below
• IP: See 2 publications below

Informations sur le produit

17115-1-AP cible ATP6V1A dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6V1A Protéine recombinante Ag10801
• Nom complet: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• Masse moléculaire calculée: 617 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC013138
• Symbole du gène: ATP6V1A
• Identification du gène (NCBI): 523
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

Publications

Species	Application	Title
human	WB	Cell; Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.; Authors - Zharko Daniloski
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins
human	WB	Nat Microbiol; The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.; Authors - Tomas Doyle
human	WB	Autophagy; Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency; Authors - Fangyang Wang
human,mouse	WB	Mol Psychiatry; Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.; Authors - A Pérez-Cañamás
human	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paulina (Verified Customer) (03-26-2025)

Fixation: 2% PFA for 20min. Permeabilization/antibody dilution solution: 0.05% saponin, 5% horse serum in PBS. Primary antibody diluted 1:100 in saponin solution for 1hr at room temperature, followed by anti-rabbit conjugated to AlexaFluor 488 (1:200 in saponin solution) for 1 hr at room temperature.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: MDA-MB-231 cells

ATP6V1A Antibody Immunofluorescence validation (1:100 dilution) in MDA-MB-231 cells (Cat no:17115-1-AP)

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