Anticorps Polyclonal de lapin anti-ATP6V1B2

• Phare
• Validé par KD/KO

ATP6V1B2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 15097-1-AP

Synonymes

ATP6B1B2, ATP6B2, ATP6V1B2, HO57, V ATPase subunit B 2, VATB, Vma2, VPP3

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Galerie de données de validation

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  WB analysis of mouse heart using 15097-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15097-1-AP

  WB result of ATP6V1B2 antibody (15097-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected HeLa cells.

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  WB analysis using 15097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15097-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15097-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15097-1-AP

  IP Result of anti-ATP6V1B2 (IP:15097-1-AP, 4ug; Detection:15097-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 15097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 15097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules K-562, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:40000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IP: See 1 publications below

Informations sur le produit

15097-1-AP cible ATP6V1B2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6V1B2 Protéine recombinante Ag7174
• Nom complet: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 56-58 kDa
• Numéro d’acquisition GenBank: BC003100
• Symbole du gène: ATP6V1B2
• Identification du gène (NCBI): 526
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).

Publications

Species	Application	Title
human	WB	Nat Microbiol; The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.; Authors - Tomas Doyle
human	WB,IP	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
pig	WB	Microorganisms; Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.; Authors - Lanxin Yuan
human	WB	J Cancer; Upregulation of V-ATPase by STAT3 Activation Promotes Anoikis Resistance and Tumor Metastasis.; Authors - Funmilayo O Adeshakin
human	WB	Vet Res; Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.; Authors - Bingxin Chu