Anticorps Monoclonal anti-ATP6V1B2

• Phare
• Validé par KD/KO

ATP6V1B2 Monoclonal Antibody for WB, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

3C11A9

N° de cat : 68441-1-Ig

Synonymes

V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2

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Galerie de données de validation

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  WB analysis using 68441-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68441-1-Ig (ATP6V1B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of A549 using 68441-1-Ig

  WB result of ATP6V1B2 antibody (68441-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected A549 cells.

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  IP experiment of HeLa using 68441-1-Ig

  IP result of anti-ATP6V1B2 (IP:68441-1-Ig, 4ug; Detection:68441-1-Ig 1:4000) with HeLa cells lysate 2000 ug.

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  FC experiment of HeLa using 68441-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug ATP6V1B2 Monoclonal antibody (68441-1-Ig, Clone:3C11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:40000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68441-1-Ig cible ATP6V1B2 dans les applications de WB, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP6V1B2 Protéine recombinante Ag7265
• Nom complet: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 56-58 kDa
• Numéro d’acquisition GenBank: BC003100
• Symbole du gène: ATP6V1B2
• Identification du gène (NCBI): 526
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).

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