Anticorps Polyclonal de lapin anti-ATP6V1F

ATP6V1F Polyclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17725-1-AP

Synonymes

ATP6S14, ATP6V1F, V ATPase 14 kDa subunit, V ATPase subunit F, V type proton ATPase subunit F, Vacuolar proton pump subunit F, VATF, Vma7

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Galerie de données de validation

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  WB analysis of MCF-7 using 17725-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17725-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17725-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17725-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17725-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17725-1-AP (ATP6V1F Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 17725-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17725-1-AP (ATP6V1F Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of MCF-7 using 17725-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug ATP6V1F antibody (17725-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HeLa, cellules Jurkat, tissu de muscle squelettique de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain, tissu pancréatique humain, tissu placentaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17725-1-AP cible ATP6V1F dans les applications de WB, IHC, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6V1F Protéine recombinante Ag12121
• Nom complet: ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
• Masse moléculaire calculée: 119 aa, 13 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC107854
• Symbole du gène: ATP6V1F
• Identification du gène (NCBI): 9296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V1F(V-type proton ATPase subunit F) is also named as ATP6S14, VATF and belongs to the V-ATPase F subunit family. It generates an electrochemical proton gradient that is acid and positive inside synaptic vesicles. ATP6V1F plays a major role as energizers of animal plasma membranes, especially apical plasma membranes of epithelial cells. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 14 kDa and 16 kDa.

Publications

Species	Application	Title
human	IHC,WB	BMC Med Genomics; ATP6V1F is a novel prognostic biomarker and potential immunotherapy target for hepatocellular carcinoma; Authors - Xinyao Hu
human	WB,CoIP	J Proteome Res; Proteomic Investigation of Differential Interactomes of Glypican 1 and a Putative Disease-Modifying Variant of Ataxia; Authors - Nur Cengiz Winter
human	WB	Front Oncol; Genome-wide association studies identify miRNA-194 as a prognostic biomarker for gastrointestinal cancer by targeting ATP6V1F, PPP1R14B, BTF3L4 and SLC7A5; Authors - Pan Huang