Anticorps Polyclonal de lapin anti-ATP6V1H

ATP6V1H Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 26683-1-AP

Synonymes

ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13

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Galerie de données de validation

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  WB analysis of mouse brain using 26683-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26683-1-AP (ATP6V1H Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 26683-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26683-1-AP (ATP6V1H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 26683-1-AP

  IP Result of anti-ATP6V1H (IP:26683-1-AP, 4ug; Detection:26683-1-AP 1:300) with mouse brain tissue lysate 3000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below

Informations sur le produit

26683-1-AP cible ATP6V1H dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6V1H Protéine recombinante Ag24688
• Nom complet: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
• Masse moléculaire calculée: 483 aa, 56 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC025275
• Symbole du gène: ATP6V1H
• Identification du gène (NCBI): 51606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB	Int J Mol Sci; Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway; Authors - Zanyan Zhao
human	WB	Front Immunol; Acidic Microenvironment Regulates the Severity of Hepatic Ischemia/Reperfusion Injury by Modulating the Generation and Function of Tregs via the PI3K-mTOR Pathway.; Authors - Xiaojie Gan
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human	WB	Biochem Biophys Res Commun; Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.; Authors - Jong-Jer Lee
rat	WB	Biophys Rep; PMID: 30596141; Authors - Min Li