Anticorps Polyclonal de lapin anti-ATP7B-Specific

ATP7B-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 19786-1-AP

Synonymes

ATP7B, ATPase, Cu++ transporting, beta polypeptide, Copper-transporting ATPase 2, EC:7.2.2.8, WC1

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Galerie de données de validation

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  WB analysis using 19786-1-AP

  Mouse liver and rat liver tissues were subjected to SDS PAGE followed by western blot with 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 19786-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ATP7B-Specific antibody (19786-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19786-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP7B-Specific antibody (19786-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

19786-1-AP cible ATP7B-Specific dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ATPase, Cu++ transporting, beta polypeptide
• Masse moléculaire calculée: 157 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_000053
• Symbole du gène: ATP7B
• Identification du gène (NCBI): 540
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.

Publications

Species	Application	Title
mouse	WB,IHC	J Immunother Cancer; C5a/C5aR pathway blocking promoted CuS-mediated cancer therapy effect by inhibiting cuproptosis resistance; Authors - Hong Yang
mouse	WB	Adv Healthc Mater; Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation; Authors - Ning Wang
human	IHC	Comput Struct Biotechnol J; Impact of Cuproptosis-related markers on clinical status, tumor immune microenvironment and immunotherapy in colorectal cancer: A multi-omic analysis; Authors - Yanfei Shao
human	WB,IHC	Gene; Integrative analysis of the cuproptosis-related gene ATP7B in the prognosis and immune infiltration of IDH1 wild-type glioma; Authors - Kun Zhang
human	IF	Medicina (Kaunas); Curcumin Effect on Copper Transport in HepG2 Cells.; Authors - Anita Berzina
rat	WB	Ann Med; Dexmedetomidine enables copper homeostasis in cerebral ischemia/reperfusion via ferredoxin 1; Authors - Qingduo Guo

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