- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-ATP8
ATP8 Polyclonal Antibody for WB, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, porc, rat
Applications
WB, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 26723-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HEK-293, cellules HepG2, cellules LNCaP, tissu cardiaque de rat |
| Résultats positifs en IF/ICC | cellules HepG2, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 44 publications below |
Informations sur le produit
26723-1-AP cible ATP8 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat
| Réactivité | Humain, porc, rat |
| Réactivité citée | Humain |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | ATP synthase 8; ATPase subunit 8 |
| Masse moléculaire calculée | 8 kDa |
| Poids moléculaire observé | 8 kDa |
| Numéro d’acquisition GenBank | YP_003024030 |
| Symbole du gène | ATP8 |
| Identification du gène (NCBI) | 4509 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
ATP8 is a subunit of F0, the functional domain of mitochondrial ATP synthase or complex V. Mutation of ATP8 has been reported as the cause of apical hypertrophic cardiomyopathy and neuropathy.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP8 antibody 26723-1-AP | Download protocol |
| IF protocol for ATP8 antibody 26723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing. | ||
Mol Cell Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma | ||
Nucleic Acids Res Selection of initiator tRNA and start codon by mammalian mitochondrial initiation factor 3 in leaderless mRNA translation | ||
Nucleic Acids Res A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis. | ||
Nucleic Acids Res A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emilie (Verified Customer) (09-24-2025) | Bands appeared sharp and well defined under the chosen dilution.
|
FH Manon (Verified Customer) (09-19-2025) | A distinct band is visible, but since it is at a very low molecular weight, it could be easily missed
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FH Baptiste (Verified Customer) (05-23-2025) | Working perfectly
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FH Mi (Verified Customer) (02-10-2023) | This antibody works very well in human adipocytes, a strong single band around 8 kd is detected when loading 10 ug total protein.
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