• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-ATP8

ATP8 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(45)

N° de cat : 26723-1-AP

Synonymes

MT-ATP8



Applications testées

Résultats positifs en WBcellules HEK-293, cellules HepG2, cellules LNCaP, tissu cardiaque de rat
Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 44 publications below

Informations sur le produit

26723-1-AP cible ATP8 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

Réactivité Humain, porc, rat
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet ATP synthase 8; ATPase subunit 8
Masse moléculaire calculée 8 kDa
Poids moléculaire observé 8 kDa
Numéro d’acquisition GenBankYP_003024030
Symbole du gène ATP8
Identification du gène (NCBI) 4509
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP8 is a subunit of F0, the functional domain of mitochondrial ATP synthase or complex V. Mutation of ATP8 has been reported as the cause of apical hypertrophic cardiomyopathy and neuropathy.

Protocole

Product Specific Protocols
WB protocol for ATP8 antibody 26723-1-APDownload protocol
IF protocol for ATP8 antibody 26723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.

Authors - Li-Sheng Zhang
humanWB

Mol Cell

Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma

Authors - Linyu Zhu
humanWB

Nucleic Acids Res

Selection of initiator tRNA and start codon by mammalian mitochondrial initiation factor 3 in leaderless mRNA translation

Authors - Muhoon Lee
humanWB

Sci Adv

Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis

Authors - Spencer D Shelton
humanWB

Nucleic Acids Res

A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.

Authors - Zidong Jia
humanWB

Nucleic Acids Res

A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.

Authors - Feilong Meng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Emilie (Verified Customer) (09-24-2025)

Bands appeared sharp and well defined under the chosen dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fibroblasts
FH

Manon (Verified Customer) (09-19-2025)

A distinct band is visible, but since it is at a very low molecular weight, it could be easily missed

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Fibroblasts
FH

Baptiste (Verified Customer) (05-23-2025)

Working perfectly

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Lymphoblastoid
ATP8 Antibody Western Blot validation (1:3000 dilution) in Lymphoblastoid (Cat no:26723-1-AP)
FH

Mi (Verified Customer) (02-10-2023)

This antibody works very well in human adipocytes, a strong single band around 8 kd is detected when loading 10 ug total protein.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: adipocytes
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