Anticorps Polyclonal de lapin anti-ATP8A1

• Phare
• Validé par KD/KO

ATP8A1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 21565-1-AP

Synonymes

ATP8A1, ATPase II, ATPASEII, ATPIA, ATPP2, Chromaffin granule ATPase II

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Galerie de données de validation

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  WB analysis of mouse brain using 21565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21565-1-AP (ATP8A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21565-1-AP (ATP8A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IF: See 3 publications below

Informations sur le produit

21565-1-AP cible ATP8A1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP8A1 Protéine recombinante Ag16071
• Nom complet: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
• Masse moléculaire calculée: 1164 aa, 131 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC109317
• Symbole du gène: ATP8A1
• Identification du gène (NCBI): 10396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	EMBO J; Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase.; Authors - Shoken Lee
human	WB, IF	Proc Natl Acad Sci U S A; AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies suppresses activation of YAP in alveolar epithelial type 2 cells.; Authors - Seunghyi Kook
mouse	WB	Sci Rep; Proteomic Analysis and Functional Characterization of P4-ATPase Phospholipid Flippases from Murine Tissues.; Authors - Jiao Wang
human	WB	Sci Rep; PPP1R12A is a recycling endosomal phosphatase that facilitates YAP activation; Authors - Chiaki Inoue ; KD Validated
human	WB	iScience; Lipid flippase dysfunction as a therapeutic target for endosomal anomalies in Alzheimer's disease.; Authors - Nanaka Kaneshiro
human	WB	Clin Proteomics; Identification of potential molecular targets for the treatment of cluster 1 human pheochromocytoma and paraganglioma via comprehensive proteomic characterization; Authors - Ondrej Vit

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mareike (Verified Customer) (01-17-2025)

The sample must not be boiled before application!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human breast cancer cell line MDA-MB-231 as wild type and Atp8a1 overexpression cell line

ATP8A1 Antibody Western Blot validation (1:1000 dilution) in human breast cancer cell line MDA-MB-231 as wild type and Atp8a1 overexpression cell line (Cat no:21565-1-AP)

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