Anticorps Polyclonal de lapin anti-ATRIP

• Phare
• Validé par KD/KO

ATRIP Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 11327-1-AP

Synonymes

AGS 1, AGS1, ATM and Rad3-related-interacting protein, ATR-interacting protein, DRN 3

---

Galerie de données de validation

• 

  WB analysis using 11327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11327-1-AP

  WB result of ATRIP antibody (11327-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATRIP transfected HeLa cells.

• 

  IP experiment of HeLa using 11327-1-AP

  IP result of anti-ATRIP (IP:11327-1-AP, 4ug; Detection:11327-1-AP 1:5000) with HeLa cells lysate 1200 ug.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules PC-3
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11327-1-AP cible ATRIP dans les applications de WB, IHC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATRIP Protéine recombinante Ag1869
• Nom complet: ATR interacting protein
• Masse moléculaire calculée: 791 aa, 86 kDa
• Poids moléculaire observé: 86-90 kDa
• Numéro d’acquisition GenBank: BC014153
• Symbole du gène: ATRIP
• Identification du gène (NCBI): 84126
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATRIP (ATR Interacting Protein) and ATR signalling complex is a central mediator of the replicative stress response (RSR),and mutations in both genes have been reported in Seckel syndrome.

Publications

Species	Application	Title
mouse	WB,IHC	Biomaterials; A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction.; Authors - Jin Qiu ; KD Validated
human	WB	Front Pharmacol; Huangkui Capsule Ameliorates Renal Fibrosis in a Unilateral Ureteral Obstruction Mouse Model Through TRPC6 Dependent Signaling Pathways.; Authors - Li-Fei Gu
mouse	WB	Biochem J; Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance; Authors - Jill E Hunter
mouse	WB	Biochem J; Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway; Authors - Jill E Hunter
human	WB	Cell Insight; ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance; Authors - Rong Luo
human	WB,CoIP	Oncogene; Phospho-TRIM21 orchestrates RPA2 ubiquitination switch to promote homologous recombination and tumor radio/chemo-resistance; Authors - Jie Zhang

{{ptg:RelatedPrimaryAntibodies}}