Anticorps Monoclonal anti-AXL

• Phare
• Validé par KD/KO

AXL Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

3D12F11

N° de cat : 68108-1-Ig

Synonymes

3D12F11, EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO

---

Galerie de données de validation

• 

  WB analysis using 68108-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68108-1-Ig (AXL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

• 

  WB analysis of HeLa using 68108-1-Ig

  WB result of AXL antibody (68108-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AXL transfected HeLa cells.

---

Applications testées

• Résultats positifs en WB: cellules NCI-H1299, cellules A431, cellules A549, cellules HeLa, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68108-1-Ig cible AXL dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AXL Protéine recombinante Ag14401
• Nom complet: AXL receptor tyrosine kinase
• Masse moléculaire calculée: 894 aa, 98 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC032229
• Symbole du gène: AXL
• Identification du gène (NCBI): 558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

{{ptg:RelatedPrimaryAntibodies}}